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Image Search Results
Journal: BMC Endocrine Disorders
Article Title: The precursor for nerve growth factor (proNGF) is not a serum or biopsy-rinse biomarker for thyroid cancer diagnosis
doi: 10.1186/s12902-019-0457-1
Figure Lengend Snippet: ProNGF serum levels after thyroidectomy and half-life. a Change in serum proNGF following total thyroidectomy . Pre- and post- thyroidectomy serum samples were available for 11 cases where pre-operative serum proNGF was detectable. No significant difference was detected between pre- and post- thyroidectomy levels of proNGF. b ProNGF in vitro half-life. Aliquots of serum negative for proNGF was spiked with 20 ng/mL recombinant proNGF dissolved in Assay Diluent A (Biosensis, Australia) in a 1:1 ratio, then incubated at 37 °C for increments of 24 h, then assayed at 1:20 dilution with Heterophilic Blocking Antibody (BL-003-1000). An exponential decay curve was fitted, giving an estimated in-vitro half-life in serum of 1.5 h. Similar results were obtained using phosphate-buffered-saline as diluent
Article Snippet: A : Effect of
Techniques: In Vitro, Recombinant, Incubation, Blocking Assay, Saline